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double up-frt  (Addgene inc)


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    Addgene inc double up-frt
    Double Up Frt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/double up-frt/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    double up-frt - by Bioz Stars, 2026-06
    90/100 stars

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    Construction and characterization of Double UP. (a) Schematic of Double UP construct. (b) Representative IUE of Double UP without Cre (top) and with pCAG-Cre in a 75:1 molar ratio (bottom). Embryos were electroporated at E14.5 and allowed 3 days to mature (E14.5+3). Scale bar, 100µm. (c) Comparison of migration of <t>mNeon-Green-</t> and <t>mScarlet-positive</t> neurons, E14.5+3. Each dot represents mean distance from the top of the cortical plate of neurons within one slice. Connected dots indicate that those measures came from the same brain. n=10 slices with 100 - 976 neurons from each slice. No significant difference between green and red populations (two-way ANOVA). Error bars not shown for clarity. (d) Comparison of reliability of controls between section matching and Double UP. Full data in . 76 comparisons made for section matching, 61 for Double UP. Only sections that had a perfect match in another brain were included for either analysis. **** p<0.0001., Kolmogorov-Smirnov t-test, two-tailed. Lines indicate mean and SD.
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    Construction and characterization of Double UP. (a) Schematic of Double UP construct. (b) Representative IUE of Double UP without Cre (top) and with pCAG-Cre in a 75:1 molar ratio (bottom). Embryos were electroporated at E14.5 and allowed 3 days to mature (E14.5+3). Scale bar, 100µm. (c) Comparison of migration of mNeon-Green- and mScarlet-positive neurons, E14.5+3. Each dot represents mean distance from the top of the cortical plate of neurons within one slice. Connected dots indicate that those measures came from the same brain. n=10 slices with 100 - 976 neurons from each slice. No significant difference between green and red populations (two-way ANOVA). Error bars not shown for clarity. (d) Comparison of reliability of controls between section matching and Double UP. Full data in . 76 comparisons made for section matching, 61 for Double UP. Only sections that had a perfect match in another brain were included for either analysis. **** p<0.0001., Kolmogorov-Smirnov t-test, two-tailed. Lines indicate mean and SD.

    Journal: bioRxiv

    Article Title: Double UP: efficient in utero electroporation via internal controls

    doi: 10.1101/571323

    Figure Lengend Snippet: Construction and characterization of Double UP. (a) Schematic of Double UP construct. (b) Representative IUE of Double UP without Cre (top) and with pCAG-Cre in a 75:1 molar ratio (bottom). Embryos were electroporated at E14.5 and allowed 3 days to mature (E14.5+3). Scale bar, 100µm. (c) Comparison of migration of mNeon-Green- and mScarlet-positive neurons, E14.5+3. Each dot represents mean distance from the top of the cortical plate of neurons within one slice. Connected dots indicate that those measures came from the same brain. n=10 slices with 100 - 976 neurons from each slice. No significant difference between green and red populations (two-way ANOVA). Error bars not shown for clarity. (d) Comparison of reliability of controls between section matching and Double UP. Full data in . 76 comparisons made for section matching, 61 for Double UP. Only sections that had a perfect match in another brain were included for either analysis. **** p<0.0001., Kolmogorov-Smirnov t-test, two-tailed. Lines indicate mean and SD.

    Article Snippet: This manuscript exclusively uses Double UP mNeon-to-mScarlet, but the others have been validated and work equally well, with different fluorescent properties. pCAG-Cre and pCAG-ERT2-Cre-ERT2 were purchased from Addgene (Addgene #13375, #13777).

    Techniques: Construct, Migration, Two Tailed Test